Sanger Sequencing | Next-Generation Sequencing | |
---|---|---|
Read Length | 500-1000bp | 50-300bp |
Degree of Dependence on Computing Tools | Low | High |
Input DNA Quantity | More | Less |
Cost Per Base | High | Low |
Time | Long | Short |
Accuracy | High | High, with errors corrected by coverage |
Throughput | Low | High |
Application |
Targeted sequencing,
validate results,
small-scale sequencing
|
Whole-genome sequencing,
large-scale sequencing
|