Sanger Sequencing vs. Next-Generation Sequencing (NGS)


Sanger Sequencing Next-Generation Sequencing
Read Length 500-1000bp 50-300bp
Degree of Dependence on Computing Tools Low High
Input DNA Quantity More Less
Cost Per Base High Low
Time Long Short
Accuracy High High, with errors corrected by coverage
Throughput Low High
Application
Targeted sequencing,
validate results,
small-scale sequencing
Whole-genome sequencing,
large-scale sequencing