| Sanger Sequencing | Next-Generation Sequencing | |
|---|---|---|
| Read Length | 500-1000bp | 50-300bp |
| Degree of Dependence on Computing Tools | Low | High |
| Input DNA Quantity | More | Less |
| Cost Per Base | High | Low |
| Time | Long | Short |
| Accuracy | High | High, with errors corrected by coverage |
| Throughput | Low | High |
| Application |
Targeted sequencing,
validate results,
small-scale sequencing
|
Whole-genome sequencing,
large-scale sequencing
|